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Updated: Mar 28, 2026

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
Next-generation sequencing yields the complete mitochondrial genome of the mummichog, Fundulus heteroclitus
Ting Zhu1,2, Shaoshu Feng1,2, Xin Liu1,2
1a College of Life Science, Liaoning Normal University , Dalian , China and.
Abstract:
Fundulus heteroclitus (Actinopteri, Cyprinodontiformes, Fundulidae), with a remarkable tolerance to osmotic stress and water temperatures, are regarded as a significant evolution model. Herein, we report the assembled complete sequence of the mummichog mitochondrial genome based on the next-generation sequencing data. The mitogenome is determined to be 16 528 bp in length and shows an organization typical of vertebrate mitochondrial genomes, including 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and 1 control region (D-loop). Overall GC content of the genome is 39.72%. Using Oryzias latipes as the outgroup, the phylogenetic analysis of 16 complete mitochondrial genomes from Cyprinodontiformes showed that F. heteroclitus together with other three Fundulus species form a cluster with strong bootstrap supports. The genus Fundulus is closely related to the genus Xenotoca.
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