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Analysis for Carom complex, signaling and function by database mining

Suxuan Liu, Xinyu Xiong, Sam Varghese Thomas

  • 1Center for Metabolic Disease Research, Department of Pharmacology, Thrombosis Research Center, hongw@temple.edu.

Frontiers in Bioscience (Landmark Edition)
|December 29, 2015
PubMed

Insights

Researchers mapped the Carom protein's tissue expression and identified 26 partners, revealing its role in signaling pathways. Lymph nodes are key tissues for Carom, which is linked to angiogenesis and stem cell generation.

Area of Science:

  • Molecular Biology
  • Cell Signaling
  • Proteomics

Background:

  • Carom is a novel protein regulating membrane curvature and pathophysiological signaling.
  • Its tissue expression, functional role, and signaling pathways remain largely uncharacterized.

Purpose of the Study:

  • To elucidate the tissue expression and signaling network of the Carom protein.
  • To establish a working model for Carom's functional implications in biological processes.

Main Methods:

  • Combined database mining strategies were employed to identify Carom partners and their expression profiles.
  • Ingenuity pathway analysis was used to identify Carom partner signaling pathways.
  • Gene expression data from the GEO database was analyzed for specific conditions.

Main Results:

  • Twenty-six Carom partners were identified, with lymph nodes identified as a Tier 1 tissue for Carom and partner expression.
  • Four conditions (hypoxia, endometriosis, PPARgamma deletion, iPSC reprogramming) altered Carom/partner expression in endothelial cells.
  • Carom/ITSN1 elevation correlated with pathological angiogenesis, while Carom/HSPA5 or UBC reduction was linked to iPSC generation.

Conclusions:

  • A Carom complex model was established, providing insights into its functional roles.
  • The study highlights Carom's involvement in both pathological angiogenesis and induced pluripotent stem cell generation.
  • Identified Carom partners and pathways offer potential targets for further research.

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