Application of high-performance liquid chromatography to enzyme activity determination

In this article some basic concepts and practical information are presented on the use of high-performance liquid chromatography (HPLC) to assay enzymatic activities. A classification of enzymatic assays is developed based on the requirements for termination and separation, and the placement of the HPLC method within this classification is discussed. The focus of the chapter is on developing a strategy for setting up assays, a discussion of some problems that must be avoided, and procedures to deal with these concerns. Some examples have been presented in Table 3, but for a more complete listing and details on the numerous activities assayed by this method ref. 1 should be consulted.