Related Experiment Videos
Planar chromatographic techniques in biomedicine: current status
A M Siouffi1, E Mincsovics, E Tyihak
1Laboratoire de Génie Chimique et Chimie Appliquée, Faculté des Sciences et Techniques de St-Jérôme, Avenue Escadrille Normandie Niémen, Marseille, France.
Journal of Chromatography
|August 11, 1989
Summary
Planar chromatography (PLC) offers versatile separation techniques, including thin-layer chromatography (TLC) and over-pressured layer chromatography. While advanced methods dominate some analyses, PLC remains crucial for lipid analysis in biomedicine.
Area of Science:
- Analytical Chemistry
- Biomedical Science
Background:
- Planar chromatography (PLC) encompasses techniques like thin-layer chromatography (TLC) and over-pressured layer chromatography (OPLC).
- These methods utilize solvent flow via capillary forces or forced-flow systems.
- Instrumentation and stationary phases are critical components.
Purpose of the Study:
- To review the available phases and instrumentation in planar chromatography.
- To examine the primary applications of PLC in biomedicine.
- To highlight the continued relevance of PLC, particularly in lipid analysis.
Main Methods:
- Examination of current phases and instrumentation in planar chromatography.
- Review of biomedical applications.
- Comparison with other chromatographic techniques.
Main Results:
- Silica gel TLC plates are predominant, but interest in alternative phases is growing.
- Unique detection methods like immunostaining are significant.
- Gas chromatography and high-performance liquid chromatography have replaced TLC for certain analyses (carbohydrates, amino acids, indole derivatives).
Conclusions:
- Planar chromatography, especially TLC, remains a valuable technique in biomedical analysis.
- Interest in PLC persists, particularly for lipid analysis, despite advancements in other chromatographic methods.