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Published on: June 30, 2017
Induction of Adipogenesis by External Volume Expansion
Jorge Lujan-Hernandez1, Luca Lancerotto, Christoph Nabzdyk
1Boston and Worcester, Mass.; and Padova, Italy From the Tissue Engineering and Wound Healing Laboratory, Division of Plastic Surgery, Brigham and Women's Hospital-Harvard Medical School; the Division of Plastic Surgery, University of Massachusetts Medical School; and the Clinic of Plastic Surgery, University of Padova.
External volume expansion using suction promotes fat cell development. This technique enhances adipogenesis, even after a single application, making it promising for fat grafting preparation.
Area of Science:
- Regenerative Medicine
- Tissue Engineering
- Adipogenesis
Background:
- External volume expansion (EVE) prepares recipient sites for fat grafting by improving tissue compliance and vascularity.
- Previous studies demonstrated EVE's pro-proliferative and angiogenic effects in a mouse model.
- Increased subcutaneous tissue thickness was noted, prompting an investigation into adipogenic potential.
Purpose of the Study:
- To assess the adipogenic potential of external volume expansion stimulation.
- To evaluate the impact of single and cyclical EVE on adipocyte formation.
Main Methods:
- A miniaturized EVE device with -25 mmHg suction was applied to mouse dorsum.
- Stimulation protocols included a single 2-hour session or 2 hours daily for 5 days.
- Tissues were analyzed for edema, inflammation, and adipocyte content (perilipin-A staining), and key gene/protein expression (PPAR-γ, Pref-1) via Western blot.
Main Results:
- Both single and cyclical EVE significantly increased adipocyte numbers (1.5- and 1.9-fold, respectively).
- Edema was observed immediately post-stimulation, followed by inflammation at 2 days.
- Peroxisome proliferator-activated receptor-γ (PPAR-γ) expression increased by the end of stimulation.
Conclusions:
- EVE, combining mechanical stretch with induced edema and inflammation, demonstrates pro-adipogenic effects.
- These pro-adipogenic effects were observed even after a single 2-hour EVE session.
- EVE shows potential for enhancing fat grafting outcomes by promoting adipogenesis.
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