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Epidemiologic marker system for Citrobacter diversus using outer membrane protein profiles.

M W Kline1, E O Mason, S L Kaplan

  • 1Department of Pediatrics, St. Louis University School of Medicine, Missouri 63104.

Journal of Clinical Microbiology
|August 1, 1989
PubMed
Summary

Outer membrane protein profiles can help track Citrobacter diversus outbreaks. This method aids in differentiating strains, improving epidemiological investigations of sepsis and meningitis in nurseries.

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Area of Science:

  • Microbiology
  • Epidemiology
  • Molecular Biology

Background:

  • Nursery outbreaks of Citrobacter diversus sepsis and meningitis lack effective epidemiologic markers.
  • Accurate strain differentiation is crucial for controlling infectious disease spread in neonatal units.

Purpose of the Study:

  • To evaluate the utility of outer membrane protein (OMP) profiles for epidemiologic differentiation of Citrobacter diversus clinical isolates.
  • To assess OMP profiling as a tool for investigating nursery outbreaks.

Main Methods:

  • Outer membrane protein profiles of Citrobacter diversus isolates were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).
  • Clinical isolates included paired samples from meningitis outbreaks, maternal-infant pairs, pneumonia cases, colonized infants, and unrelated strains.

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  • Biotyping was used in conjunction with OMP profiling for enhanced differentiation.

    • Main Results:

    • Eleven distinct OMP profiles were identified based on the presence or absence of five specific outer membrane proteins.
    • Epidemiologically related isolates showed complete concordance in their OMP profiles.
    • Unrelated epidemic strains exhibited distinct OMP profiles, differentiating them from each other and from non-epidemic strains.
    • Combined OMP profiling and biotyping differentiated five epidemic strains from most unrelated isolates.

    Conclusions:

    • Outer membrane protein profiling is a valuable method for the epidemiologic investigation of Citrobacter diversus.
    • This technique can aid in tracing the source and spread of C. diversus infections, particularly in nursery settings.
    • OMP analysis, complemented by biotyping, offers a robust approach to strain differentiation for outbreak investigations.