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Updated: Mar 28, 2026

Assessment of Chemical Toxicity in Adult Drosophila Melanogaster
Published on: March 24, 2023
[The chemical toxicological study of cyclomed]
D B Fedorov1, A V Kireeva2, Yu K Chikhvatova1
1St.-Petersburg State Chemicopharmaceutical Academy, Russian Ministry of Health, St.-Petersburg, Russia, 193376.
Abstract:
The objective of the present study was to develop the methods for the chemical and toxicological analysis of cyclopentolate contained in the material evidence for the purpose of forensic chemical and forensic toxicological expertises. The optimal conditions for the isolation of cyclopentolate from the cyclomed preparation and biological fluids were created using a chloroform-2-ptropanol mixture at pH of the medium 10.0. The substance of interest was identified with the use of the color and flocculation reactions, UV spectroscopy, thin layer chromatography and gas chromatography with the use of a mass-selective detector. HPLC and GCh/MS were employed for the quantitative determination. The results of the two methods are in excellent agreement.
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