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Updated: Mar 28, 2026

Lethality Bioassay Using Artemia salina L.
Published on: October 11, 2022
[Algicidal effect of (2-isobutoxyphenyl) amine on Alexandrium tamarense]
Objective:
A strain named BS01 showed strong algicidal activity to Alexandrium tamarense and we got algicidal compound (2-isobutoxyphenyl) amine from BS01 to study its algicidal effect on A. tamarense.
Methods:
We studied the algicidal mechanism of (2-isobutoxyphenyl) amine on photosynthetic process, antioxidant enzyme activities and morphological change of A. tamarense.
Results:
After 24 hours treatment with (2-isobutoxyphenyl) amine, algicidal activity was 84. 1% with the concentration of 20 µg/mL. The compound could induce a reactive oxygen species burst in P. globosa in 0. 5 hours which could cause serious oxidative damage to algal cells. The Fv/Fm value which could reflect photosystem II (PS II) electron flow status also decreased. To eliminate the excess ROS, the activities of the antioxidant systems (including superoxide dismutase and catalase) increased significantly during exposure. Transmission electron microscope analysis showed obvious morphological modifications of chloroplast dismantling as a part of the algicidal process.
Conclusion:
These results indicated that the lysis mechanism of algicidal compound on algae may primarily be the increasing level of ROS in the algal cells.
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