Related Experiment Video
Updated: Mar 28, 2026

Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
Published on: September 9, 2012
[Correlation of three common variation loci in von Willebrand factor gene and pulmonary thromboembolism disease]
Yuejie Sun1, Xiaomao Xu, Liang Sun
1Peking University Fifth School of Clinical Medicine, Cell Laboratory, Beijing Institute of Geriatric Medicine, Beijing Hospital, Beijing 100730, China.
Objective:
To elucidate the relationship between three common variation loci of von Willebrand factor (VWF) gene (rs216321, rs216325 and rs1800378) and pulmonary thromboembolism.
Methods:
A total of 95 patients with definite pulmonary thromboembolism (PTE) at Beijing Chaoyang Hospital and Beijing Hospital during November 2008 to March 2012 served as PTE group while 90 healthy subjects at Beijing Hospital during the same period as control group. Fasting venous blood samples were collected for extracting genomic DNA. Three common variation loci with single nucleotide polymorphism were rs216321 (T/C), rs216325 (G/A) and rs1800378 (T/C) and their minor allele frequency was over 0.05 in VWF gene. The method of polymerase chain reaction (PCR)-Sanger was employed for sequencing. The differences of alleleic and genotypic frequencies between PTE and control groups were compared for each locus. And the correlations of their haplotypes with PTE were analyzed.
Results:
The distributions of rs216325 (G/A) and rs1800378 (T/C) in VWF gene had significant difference between PTE and control groups (P=0.039, 0.006). And rs216325 with genotype AA was positively correlated with PTE occurrence (r=1.914, P=0.047). And rs1800378 with genotype TT was also positively correlated with PTE occurrence (r=0.282, P=0.008). The distributions of haplotype TGT had significant differences between PTE and control groups. This haplotype was positively correlated with PTE occurrence (r=0.239, P<0.001).
Conclusions:
The rs216325 and rs1800378 loci variations in VWF gene are associated with PTE, rs216325 with genotype AA and rs1800378 with genotype TT. And haplotype TGT indicates a high risk of PTE onset.
More Related Videos
08:30Investigating von Willebrand Factor Pathophysiology Using a Flow Chamber Model of von Willebrand Factor-platelet String Formation
Published on: August 14, 2017
18:30RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
Published on: February 13, 2013
Related Concept Videos
Venous Thrombosis I: Introduction
Venous Thrombosis II: Clinical Manifestations and Diagnostic Studies
Pharmacogenetic Phenotypes: Alterations in Pharmacokinetics, Drug Targets and Biologic Milieu
Genome-wide Association Studies-GWAS
GWAS does not require the identification of the target gene involved in...
Disorders of Hemostasis
Thromboembolic Disorders
Two factors primarily cause thromboembolic conditions.
Venous Thrombosis III: Interprofessional Care