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Updated: Mar 28, 2026

A Method for Targeted 16S Sequencing of Human Milk Samples
Published on: March 23, 2018
Human Milk Bacterial and Glycosylation Patterns Differ by Delivery Mode
Marina Hoashi1, Lawrence Meche2, Lara K Mahal2
1Bioinformatics Program, New York University Tandon School of Engineering, New York, NY, USA Division of Translational Medicine, New York University School of Medicine, New York, NY, USA.
Abstract:
Mammals have evolved to nourish their offspring exclusively with maternal milk for around half of the lactation period, a crucial developmental window. In view of oral-breast contact during lactation and the differences in oral microbiota between cesarean section (C-section) and vaginally delivered infants, we expected differences in milk composition by delivery mode. We performed a cross-sectional study of banked human milk and found changes related to time since delivery in bacterial abundance and glycosylation patterns only in milk from women who delivered vaginally. The results warrant further research into the effects of delivery mode on milk microbes, milk glycosylation, and postpartum infant development.
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