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Updated: Mar 28, 2026
Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants
Published on: January 26, 2019
[Study of immunomodulatory function of exosomes derived from human umbilical cord mesenchymal stem cells]
Ming Liu1, Jinsong Wang, Muyun Liu
1State Key Laboratory of Respiratory Diseases, Guangzhou Institute of Respiratory Diseases, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou 510120, China.
Objective:
To investigate the immumodulation ability of exosomes secreted from human umbilical cord-derived mesenchymal stem cells (hUC-MSCs).
Methods:
hUC-MSCs were isolated and cultured.Exosomes were isolated from the culture media of the third-generation hUC-MSCs. The expression of specific surface marker CD9 and CD81 were detected by Western blot, and the concentration of hUC-MSCs exosomes(hUC-MSCs-ex) was evaluated by BCA assay. CD3/CD28-stimulated peripheral blood mononuclear cells(PBMCs) from healthy donor were co-cultured with different concentration of hUC-MSCs-ex for 72 h. The percentage of Th17 and Treg cells and the proliferation of CD4⁺ and CD8⁺ T cells were detected by flow cytometry. ELISA was used to test the level of IFN-γ, IL-6, TNF-α and TGF-β1.
Results:
hUC-MSCs-ex inhibited the proliferation of CD4⁺ and CD8⁺ cells obviously,and increased the proportion of CD4⁺ CD25⁺ FoxP3⁺ Treg cells, with high expression of CD81 and CD9. After CD3/CD28 monoclonal antibody stimulated, the percentage of CD45⁺ CD4⁺ Ki67⁺ and CD45⁺ CD8⁺ Ki67⁺ cells were 85.3% ± 5.6% and 72.6% ± 6.3%, respectively. Meanwhile, the level of TGF-β1 were elevated and the level of IFN-γ, IL-6 and TNF-α were decreased (P<0.05).
Conclusion:
hUC-MSCs-ex has the immunomodulatory functionin vitro, which could be a new therapeutic agent for the treatment of immune disorders.
Insights
Human umbilical cord-derived mesenchymal stem cell exosomes (hUC-MSCs-ex) show immunomodulatory effects. These exosomes inhibit T cell proliferation and promote regulatory T cell development, suggesting potential for treating immune disorders.
Area of Science:
- Immunology
- Stem Cell Biology
- Exosome Research
Background:
- Mesenchymal stem cells (MSCs) are known for their immunomodulatory properties.
- Exosomes, secreted vesicles from cells, carry bioactive molecules and mediate intercellular communication.
- Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) are a promising source for therapeutic applications.
Purpose of the Study:
- To investigate the immunomodulatory potential of exosomes derived from hUC-MSCs (hUC-MSCs-ex).
- To assess the effect of hUC-MSCs-ex on T cell proliferation and differentiation in vitro.
- To determine the impact of hUC-MSCs-ex on cytokine profiles related to immune responses.
Main Methods:
- Isolation and characterization of exosomes from hUC-MSC culture supernatants.
- Co-culture of peripheral blood mononuclear cells (PBMCs) with varying concentrations of hUC-MSCs-ex.
- Flow cytometry analysis to quantify T cell populations (Th17, Treg) and proliferation markers (Ki67).
- ELISA to measure levels of key cytokines (IFN-γ, IL-6, TNF-α, TGF-β1).
Main Results:
- hUC-MSCs-ex demonstrated significant inhibition of CD4+ and CD8+ T cell proliferation.
- An increased proportion of regulatory T cells (Treg) was observed in the presence of hUC-MSCs-ex.
- Cytokine analysis revealed elevated TGF-β1 levels and decreased levels of IFN-γ, IL-6, and TNF-α.
Conclusions:
- hUC-MSCs-ex possess in vitro immunomodulatory capabilities.
- The findings suggest that hUC-MSCs-ex can suppress excessive immune responses.
- hUC-MSCs-ex represent a potential novel therapeutic agent for immune-related disorders.
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