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Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung
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Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung

Published on: June 5, 2012

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Pseudomonas aeruginosa antibiotic resistance in Australian cystic fibrosis centres

Daniel J Smith1,2,3, Kay A Ramsay4,5, Stephanie T Yerkovich2,6

  • 1The Adult Cystic Fibrosis Centre, The Prince Charles Hospital, Brisbane, Queensland, Australia.

Respirology (Carlton, Vic.)
|December 30, 2015
PubMed
Abstract

Insights

Treatment intensity and location in Australian cystic fibrosis (CF) centers correlate with multi-antibiotic resistant Pseudomonas aeruginosa (MARPA) risk. However, these factors did not worsen clinical outcomes in CF patients.

Area of Science:

  • Medical Microbiology
  • Infectious Diseases
  • Clinical Medicine

Background:

  • Chronic Pseudomonas aeruginosa infection significantly increases morbidity, antibiotic use, and mortality in cystic fibrosis (CF) patients.
  • Understanding factors influencing antibiotic resistance is crucial for managing CF.
  • This study links Australian CF registry data with microbiological data to investigate treatment delivery and resistance patterns.

Purpose of the Study:

  • To examine the association between the location and intensity of treatment delivery and Pseudomonas aeruginosa antibiotic resistance in Australian CF patients.
  • To identify factors predicting antibiotic resistance and treatment intensity across different CF centers.
  • To compare treatment practices and antibiotic resistance prevalence between Australian CF centers.

Main Methods:

  • Sputa samples were collected from pediatric and adult CF patients across 18 Australian CF centers.
  • Antibiotic susceptibility testing of P. aeruginosa isolates was performed, and data were correlated with clinical characteristics and treatment intensity.
  • Differences in treatment and antibiotic resistance were compared between centers.

Main Results:

  • Significant variations in antibiotic usage, maintenance treatments, and multi-antibiotic resistant P. aeruginosa (MARPA) prevalence were observed among Australian CF centers.
  • In pediatric centers, MARPA correlated with intravenous antibiotic use and the Australian state of treatment.
  • In adult centers, BMI and treating state were associated with MARPA, with intravenous antibiotic use predicted by multiple factors including BMI and azithromycin.

Conclusions:

  • Increased treatment intensity in pediatric CF centers and the Australian state of treatment are linked to a higher risk of MARPA.
  • Despite associations with MARPA, these treatment-related factors did not correlate with worse clinical outcomes in CF patients.
Keywords:
Pseudomonas aeruginosaantibiotic resistancecystic fibrosisepidemiologytreatment

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