Terminal Bacteroid Differentiation Is Associated With Variable Morphological Changes in Legume Species Belonging to
Jesús Montiel1, Attila Szűcs1, Iulian Z Boboescu1,2
11 Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Temesvári krt. 62, 6726 Szeged, Hungary;
Molecular Plant-Microbe Interactions : MPMI
|December 30, 2015
Summary
Legumes in the inverted repeat-lacking clade (IRLC) induce terminal differentiation in their bacterial symbionts. This process, likely mediated by NCR peptides, results in altered bacteroid development across various legume species.
Area of Science:
- Plant-microbe interactions
- Molecular biology
- Legume symbiosis
Background:
- Legumes of the inverted repeat-lacking clade (IRLC) induce terminal differentiation in their bacterial endosymbionts.
- This differentiation involves genome endoreduplication, cell enlargement, and loss of division capacity.
- Nodule-specific cysteine-rich (NCR) peptides are implicated as plant effectors in this process.
Purpose of the Study:
- To investigate the fate of bacteroids in various IRLC legume species beyond Medicago.
- To determine if terminal differentiation occurs across different genera within the IRLC.
- To explore the role of NCR peptides in bacteroid differentiation.
Main Methods:
- Comparative analysis of bacteroid morphology and DNA content across multiple IRLC legume species.
- Assessment of bacteroid reproductive ability.
- Bioinformatic analysis to identify NCR genes in available IRLC legume sequences.
Main Results:
- Bacteroids from all tested IRLC legumes showed increased size and DNA content compared to cultured cells.
- Variable degrees of cell elongation were observed among different species.
- Bacteroids exhibited significantly reduced reproductive ability.
- NCR genes were present in all IRLC species with available sequence data.
Conclusions:
- IRLC legumes consistently induce terminal differentiation in their bacterial endosymbionts.
- This differentiation results in diverse bacteroid morphotypes across species.
- NCR peptides are likely key mediators of bacteroid terminal differentiation in IRLC legumes.
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