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Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor
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Perchlorate reduction by hydrogen autotrophic bacteria and microbial community analysis using high-throughput
Dongjin Wan1, Yongde Liu1, Zhenhua Niu1
1School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, Henan, 450001, China.
Biodegradation
|December 31, 2015
Summary
Hydrogen-driven perchlorate reduction is effective and safe for drinking water. This study reveals microbial community shifts and identifies Thauera as the dominant bacteria during acclimation.
Area of Science:
- Environmental microbiology
- Water treatment technologies
- Bioremediation
Background:
- Perchlorate reduction using hydrogen autotrophic bacteria offers high efficiency and is safe for drinking water.
- Limited information exists on microbial community structure and dominant bacteria during perchlorate-reducing bacteria acclimation.
Purpose of the Study:
- To investigate changes in microbial community structure and biodiversity during the acclimation of perchlorate-reducing hydrogen autotrophic bacteria.
- To identify dominant perchlorate-reducing bacteria (PRB) and understand their metabolic preferences.
Main Methods:
- Acclimation of perchlorate-reducing hydrogen autotrophic bacteria from activated sludge using hydrogen aeration.
- Application of high-throughput sequencing to analyze microbial community dynamics.
- Kinetic analysis using the Michaelis-Menten model.
Main Results:
- The Michaelis-Menten model accurately described perchlorate reduction kinetics (q(max) = 2.521-3.245 mg/gVSS h, K(s) = 5.44-8.23 mg/l).
- Optimal perchlorate reduction occurred at pH 9.0 within a range of 5.0-11.0.
- Enriched bacteria preferred nitrate over perchlorate and sulfate as electron acceptors.
- Biodiversity decreased significantly during acclimation, with stabilization after 9 cycles.
- The Thauera genus (Rhodocyclales) emerged as the dominant PRB.
Conclusions:
- Hydrogen autotrophic reduction is a viable method for perchlorate removal.
- Microbial community structure significantly changes during acclimation, with Thauera being the key PRB.
- Understanding these microbial dynamics is crucial for optimizing bioremediation strategies.
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