Related Experiment Video
Updated: Mar 28, 2026
12:40
Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System
Published on: December 7, 2012
29.7K
Hemostatic Efficacy of Pathogen-Inactivated Blood Components
1Department of Pathology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.
Seminars in Thrombosis and Hemostasis
|December 31, 2015
Summary
Pathogen inactivation technologies reduce transfusion risks for plasma and platelets. While generally effective, some studies show minor impacts on clotting factors and platelet counts.
Area of Science:
- Transfusion Medicine
- Blood Product Safety
- Infectious Disease Control
Background:
- Pathogen inactivation (PI) technologies mitigate infectious risks associated with plasma and platelet transfusions.
- These technologies can impact the functional integrity of blood components, including clotting factors and platelet viability.
Purpose of the Study:
- To review the effects of pathogen inactivation on plasma and platelet transfusions.
- To assess the clinical efficacy and safety of PI technologies in transfusion medicine.
Main Methods:
- Review of randomized clinical trials (RCTs) and studies on plasma treated with solvent-detergent, methylene blue, amotosalen (AM), or riboflavin (RF) plus UV light.
- Analysis of RCTs comparing pathogen-reduced platelets (treated with UV plus RF or AM) versus untreated platelets.
Main Results:
- PI plasma retains 75-85% of clotting factor activity and is generally comparable to untreated plasma in clinical outcomes, with one exception for prothrombin time correction with RF plasma.
- PI platelets show reduced 1-hour corrected count increments (median 75% of untreated) and shorter lifespans (4-5 days vs. 6-7 days).
- Clinical bleeding outcomes for PI platelets are largely equivalent, with one study noting more bleeding with AM platelets.
Conclusions:
- Pathogen inactivation effectively reduces transfusion-associated infectious risks for plasma and platelets.
- While PI technologies have minor effects on component quality and function, they are generally safe and effective in clinical practice.
- Ongoing research includes UVC light treatment for pathogen inactivation of platelets.
Related Concept Videos
01:20Extrinsic and Intrinsic Pathways of Hemostasis
15.2K
Blood clotting or coagulation involves extrinsic and intrinsic pathways, which ultimately merge into the common pathway, forming a fibrin clot.
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which...
The Extrinsic Pathway
The extrinsic pathway of coagulation is typically initiated by tissue damage that exposes blood to tissue factor (TF), a protein released by the damaged tissue cells outside the blood vessels—this interaction with TF triggers biochemical reactions involving specific clotting factors. The key player here is Factor VII, which...
15.2K
01:30Anticoagulant Drugs: Low-Molecular-Weight Heparins
2.3K
Hemostasis is a crucial process that prevents excessive blood loss from damaged blood vessels. It involves various mechanisms such as vasoconstriction, platelet adhesion and activation, and fibrin formation. The importance of each mechanism depends on the type of vessel injury. In contrast, thrombosis is the abnormal formation of a blood clot within the blood vessels, leading to potential complications if the clot obstructs blood flow. Thrombosis can be caused by increased coagulability of the...
2.3K
01:05Introduction to Hemostasis
16.3K
Hemostasis is a complex physiological process that prevents excessive bleeding when a blood vessel is injured. It's crucial for maintaining the integrity of the circulatory system, as it ensures that our blood remains fluid while still within the vascular network and yet clots to prevent blood loss upon vessel injury.
The three phases of hemostasis involve many clotting factors present in plasma and several substances released by platelets and injured tissue cells. It is a fast, localized,...
The three phases of hemostasis involve many clotting factors present in plasma and several substances released by platelets and injured tissue cells. It is a fast, localized,...
16.3K
01:24Disorders of Hemostasis
2.7K
Hemostasis, the process that stops bleeding after a blood vessel injury, is crucial for maintaining the integrity of the circulatory system. However, disorders of hemostasis can disrupt this delicate balance, leading to either excessive clotting or bleeding. These disorders can be broadly classified into thromboembolic disorders and bleeding disorders.
Thromboembolic Disorders
Two factors primarily cause thromboembolic conditions.
Thromboembolic Disorders
Two factors primarily cause thromboembolic conditions.
2.7K
02:45Blood Transfusion and Agglutination
15.7K
Blood transfusion is a therapeutic measure to restore the blood volume after extensive blood loss due to an accident or a medical procedure. Blood transfusion involves drawing a certain amount of blood from a suitable donor and infusing it into the recipient.
History
The history of blood transfusion dates back to the 17th century, when early attempts were made in animals. In 1818 James Blundell, a British doctor, performed the first successful human blood transfusion. Later in 1900, Karl...
History
The history of blood transfusion dates back to the 17th century, when early attempts were made in animals. In 1818 James Blundell, a British doctor, performed the first successful human blood transfusion. Later in 1900, Karl...
15.7K
01:22Formation of the Platelet Plug
10.7K
The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
10.7K