[Primary candidate rna biomarker screening by RNA-seq for prostate cancer diagnostics]

Area of Science:

• Molecular Biology
• Oncology
• Biomarker Discovery

Context:

• Prostate cancer diagnosis often relies on invasive procedures.
• Minimally invasive methods for detecting prostate cancer biomarkers are highly desirable.
• RNA biomarkers in bodily fluids offer a promising avenue for noninvasive screening.

Purpose:

• To evaluate the feasibility of using RNA sequencing (RNA-seq) to screen for prostate cancer RNA biomarkers in plasma and urine.
• To assess the presence of known prostate cancer RNA biomarkers in samples from patients with benign prostatic hyperplasia (BPH).
• To investigate the influence of library preparation methods on biomarker detection.

Summary:

• RNA-seq is a feasible approach for screening prostate cancer RNA biomarkers in plasma and urine.
• Significant numbers of prostate cancer-associated RNA biomarkers were detected in both plasma and urine samples from BPH patients.
• The number of detected RNA biomarkers varied depending on the library preparation method used for transcriptome profiling.
• The presence of these biomarkers in BPH samples indicates a potential lack of specificity, necessitating further investigation.

Impact:

• Demonstrates the potential of RNA-seq in plasma and urine for minimally invasive prostate cancer diagnostics.
• Highlights the need for rigorous validation to ensure the specificity of identified RNA biomarkers.
• Suggests that RNA biomarker profiles may differ between prostate cancer and BPH, requiring further research to differentiate.