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Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins
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Methods to Identify the NMR Resonances of the 13C-Dimethyl N-terminal Amine on Reductively Methylated Proteins

Published on: December 12, 2013

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Identification of activators of methionine sulfoxide reductases A and B

Predrag Cudic1, Neelambari Joshi2, Daphna Sagher2

  • 1Torrey Pines Institute for Molecular Studies, Port St. Lucie, Florida, USA.

Biochemical and Biophysical Research Communications
|January 1, 2016
PubMed

Insights

New compounds activate methionine sulfoxide reductase (Msr) enzymes, which protect cells from oxidative damage. This discovery offers a potential strategy for slowing aging and treating age-related diseases.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Gerontology

Background:

  • Methionine sulfoxide reductase (Msr) enzymes, MsrA and MsrB, repair oxidative damage to proteins.
  • MsrA has been linked to lifespan extension in model organisms like Drosophila melanogaster and Caenorhabditis elegans.
  • Activating cellular protective mechanisms against oxidative stress is a potential therapeutic approach for age-related diseases.

Purpose of the Study:

  • To identify novel compounds that activate Msr enzymes.
  • To investigate the potential of these compounds in modulating cellular oxidative damage repair pathways.

Main Methods:

  • Screening of compounds structurally related to natural product fusaricidins.
  • Assaying the activation of recombinant bovine and human MsrA and human MsrB enzymes.

Main Results:

  • Identification of novel compounds that significantly activate both MsrA and MsrB.
  • Demonstration of marked activation of recombinant bovine and human MsrA and human MsrB by these compounds.

Conclusions:

  • Compounds structurally related to fusaricidins can activate Msr enzymes.
  • These activators represent a new class of molecules with potential therapeutic applications in aging and oxidative stress-related diseases.
Keywords:
Fusaricidin analoguesMethionine sulfoxide reductasesMsr activation

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