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Preclinical Assessment of a Fully Automated Multiplex PCR Panel for Detection of Central Nervous System Pathogens
K E Hanson1, E S Slechta2, J A Killpack3
1University of Utah Department of Medicine, Division of Infectious Diseases, School of Medicine, Salt Lake City, Utah, USA University of Utah Department of Pathology, Clinical Microbiology, Salt Lake City, Utah, USA kim.hanson@hsc.utah.edu anne.blaschke@hsc.utah.edu.
Abstract:
We evaluated a multiplexed PCR panel for the detection of 16 bacterial, viral, and fungal pathogens in cerebrospinal fluid. Panel results were compared to routine testing, and discrepancies were resolved by additional nucleic acid amplification tests or sequencing. Overall, the positive and negative agreements across methods were 92.9% and 91.9%, respectively.
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