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Flow Cytometry To Assess Cerebrospinal Fluid Fungal Burden in Cryptococcal Meningitis

James E Scriven1, Lisa M Graham2, Charlotte Schutz3

  • 1Liverpool School of Tropical Medicine, Liverpool, Merseyside, United Kingdom Wellcome Trust Liverpool Glasgow Centre for Global Health Research, Liverpool, United Kingdom Clinical Infectious Diseases Research Initiative, Institute of Infectious Disease and Molecular Medicine, University of Cape Town, Cape Town, South Africa Department of Medicine, University of Cape Town and Groote Schuur Hospital, Cape Town, South Africa jscriven@liv.ac.uk.

Journal of Clinical Microbiology
|January 1, 2016
PubMed

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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