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Updated: Mar 28, 2026

Identification of Circular RNAs using RNA Sequencing
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Characterization of Circular RNAs.

Yang Zhang1, Li Yang2, Ling-Ling Chen3,4

  • 1State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.

Methods in Molecular Biology (Clifton, N.J.)
|January 2, 2016
PubMed
Summary

This study presents a detailed pipeline for identifying and validating circular RNAs (ribonucleic acids), which have unique closed-loop structures. The method effectively characterizes both circular intronic RNAs (ciRNAs) and exonic circular RNAs (circRNAs) in humans.

Keywords:
Circular RNAsRNA fractionationRNase RciRNAscircRNAs

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Area of Science:

  • Molecular Biology
  • Genomics
  • RNA Biology

Background:

  • Circular RNAs (ribonucleic acids) are increasingly recognized as significant RNA species across diverse organisms, from fruit flies to humans.
  • Unlike linear RNAs, circular RNAs possess a unique covalently closed loop structure, lacking 5' caps and 3' tails.
  • This distinct structure necessitates specialized techniques for accurate identification and validation.

Purpose of the Study:

  • To develop and present a comprehensive pipeline for the characterization of circular RNAs.
  • To validate the pipeline's efficacy in identifying and analyzing different types of circular RNAs.

Main Methods:

  • Development of a detailed bioinformatic and experimental pipeline tailored for circular RNA identification.
  • Application of the pipeline to analyze circular intronic RNAs (ciRNAs) derived from intron lariats.
  • Utilizing the pipeline to study circular RNAs produced from back-spliced exons (circRNAs) in human samples.

Main Results:

  • The described pipeline enables robust identification and characterization of circular RNAs.
  • Successful application of the pipeline to human samples for studying ciRNAs and circRNAs.
  • Demonstration of the pipeline's utility in validating these unique RNA structures.

Conclusions:

  • The developed pipeline provides a reliable method for circular RNA characterization.
  • This methodology facilitates further research into the biogenesis and function of ciRNAs and circRNAs.
  • The pipeline is a valuable tool for researchers investigating circular RNA populations in transcriptomes.