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Differential Gene Expression in Coiled versus Flow-Diverter-Treated Aneurysms: RNA Sequencing Analysis in a Rabbit
A Rouchaud1, C Johnson2, E Thielen2
1From the Applied Neuroradiology Laboratory (A.R., C.J., E.T., D.S., Y.-H.D., D.D., W.B., D.F.K., R.K.) Rouchaud.Aymeric@mayo.edu aymeric.rouchaud@gmail.com.
AJNR. American Journal of Neuroradiology
|January 2, 2016
Summary
RNA sequencing reveals distinct molecular responses to aneurysm treatments. Flow diverters promote endothelialization but may increase rupture risk, while coils enhance wound healing but could lead to recurrence.
Area of Science:
- Vascular biology
- Genomics
- Biomaterials science
Background:
- Mechanisms of aneurysm healing and rupture after endovascular treatment are unclear.
- RNA sequencing can identify key genes and pathways involved in aneurysm response.
Purpose of the Study:
- To investigate molecular differences between flow diverter and coil treatments for aneurysms.
- To identify genes and pathways related to aneurysm healing and potential complications.
Main Methods:
- Elastase-induced rabbit aneurysms were treated with coils or flow diverters, or left untreated.
- RNA sequencing was performed on aneurysm tissues 4 weeks post-treatment.
- Ingenuity Pathway Analysis was used to analyze gene expression data.
Main Results:
- Significant differential gene expression was observed between treatment groups and controls.
- Flow diverters showed overexpression of apelin (endothelialization) and Keratin 8 (potential rupture risk).
- Coils demonstrated overexpression of wound healing genes (dectin 1, hedgehog interacting protein) and metallopeptidases.
Conclusions:
- Endovascular treatments elicit distinct molecular responses in aneurysms.
- Flow diverters may promote endothelialization but carry a risk of delayed rupture.
- Coils promote wound healing but may be associated with aneurysm recurrence.
