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Published on: December 28, 2015
Effect of Eudragit on In Vitro Transfection Efficiency of PEI-DNA Complexes
Naveen Kanthamneni1, Bryant Yung2, Robert J Lee3
1Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University, Columbus, OH, U.S.A.
Aim:
Eudragit® E 100 (EE100) was used to improve the transfection efficiency of polyethylenimine (PEI).
Materials And Methods:
Mobility of PEI-DNA complexes with and without EE100 were visualized by agarose gel electrophoresis and their transfection efficiencies were investigated in KB human oral carcinoma cells by flow cytometry. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay was used to determine the viability of transfected cells.
Results:
Gel electrophoresis illustrated formation of complete complexes at N/P ratios above 5. PEI had the highest transfection efficiency at an N/P ratio of 15, whereas in combination with EE100, the transfection efficiency was highest at an N/P ratio of 7.5. High concentrations of EE100 in combination with PEI were found to reduce cell viability.
Conclusion:
The results show a synergistic action of EE100 in transfection of DNA at low N/P ratios compared to PEI alone.
