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Controlling Cooperativity in β-Cyclodextrin-DNA Binding Reactions.

P S Alves1, O N Mesquita1, M S Rocha2

  • 1Departamento de Física, Universidade Federal de Minas Gerais , Av. Antônio Carlos, 6627, Belo Horizonte, Minas Gerais 31270-901, Brazil.

The Journal of Physical Chemistry Letters
|January 2, 2016
PubMed
Summary
This summary is machine-generated.

We studied how neutral beta-cyclodextrin (CD) interacts with DNA using single-molecule stretching. We found CD can cluster and denature DNA, with effects tunable by CD surface charge.

Keywords:
DNAcooperative bindingcyclodextrinpersistence length

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Area of Science:

  • Biophysics
  • Supramolecular Chemistry
  • Molecular Biology

Background:

  • Cyclodextrins (CDs) are known to interact with DNA.
  • Understanding these interactions is crucial for DNA-based applications.

Purpose of the Study:

  • To investigate the mechanical properties of beta-cyclodextrin (CD)-DNA complexes.
  • To elucidate the mechanisms of CD-DNA interaction.
  • To determine physicochemical parameters of the interaction.

Main Methods:

  • Single-molecule stretching experiments using optical tweezers.
  • Application of a quenched disorder statistical model.
  • Varying beta-cyclodextrin (CD) concentration.

Main Results:

  • Mechanical properties of CD-DNA complexes were monitored as a function of CD concentration.
  • Equilibrium binding constants and cooperativity were extracted using a statistical model.
  • Two interaction mechanisms were identified: CD clustering and local DNA denaturation via hydrogen bonding.

Conclusions:

  • Beta-cyclodextrin (CD) interacts with DNA through clustering and denaturation.
  • The cooperativity of this interaction can be controlled by modifying the surface charge of beta-cyclodextrin (CD).
  • The findings provide insights into supramolecular interactions with DNA.