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Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures
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Protein turnover during in vitro tissue engineering.

Qiyao Li1, Zhen Chang2, Gisele Oliveira2

  • 1Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.

Biomaterials
|January 3, 2016
PubMed
Summary

Researchers developed a new method to track protein changes during tissue regeneration. This technique quantifies how cells remodel biological scaffolds, advancing tissue engineering and organ repair strategies.

Keywords:
Acellular scaffoldProtein turnoverReseedingStable isotope labelingTissue remodelingVocal fold fibroblastVocal fold mucosa

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Proteomics

Background:

  • Regenerating tissues and organs involves repopulating acellular scaffolds with cells.
  • Cells are expected to remodel scaffolds via protein synthesis and degradation.
  • The dynamics of this in vitro remodeling process are not well understood.

Purpose of the Study:

  • To develop and validate a technique for measuring dynamic proteome changes during in vitro tissue remodeling.
  • To quantify protein turnover and scaffold remodeling by reseeded cells.
  • To apply this technique to the vocal fold mucosa model system.

Main Methods:

  • Optimized decellularization and recellularization protocols for vocal fold mucosa.
  • Developed a stable isotope labeling strategy to distinguish scaffold proteins from newly synthesized proteins.
  • Utilized mass spectrometry-based proteomics to analyze dynamic proteome changes.

Main Results:

  • Successfully differentiated and quantified remnant scaffold proteins versus newly synthesized cellular proteins.
  • Elucidated the turnover rates of specific matrix and cellular proteins during remodeling.
  • Demonstrated the impact of cell-scaffold interactions on proteome dynamics.

Conclusions:

  • The developed technique enables dynamic proteome analysis during in vitro tissue remodeling.
  • Provides novel insights into the molecular mechanisms of scaffold remodeling by cells.
  • This method is broadly applicable to various tissue and organ engineering applications.