Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.6K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.6K
CRISPR01:59

CRISPR

59.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
59.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Remuscularizing the Failing Human Heart.

The New England journal of medicine·2026
Same author

Leveraging human genetic variation to therapeutically target hundreds of genes with dominant & dispensable disease alleles.

medRxiv : the preprint server for health sciences·2026
Same author

Using image classifiers to predict CMT2A disease-relevant mitochondrial motility phenotypes in iPSC motor neurons.

bioRxiv : the preprint server for biology·2026
Same author

Author Correction: Myocardial reprogramming by HMGN1 underlies heart defects in trisomy 21.

Nature·2026
Same author

Genome-wide CRISPRi screen identifies basigin loss as protective in cardiac hypoxia.

bioRxiv : the preprint server for biology·2026
Same author

Monitoring biological effects of somatic cell genome editing.

Nature reviews. Genetics·2026

Related Experiment Video

Updated: Mar 28, 2026

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

8.9K

Efficient CRISPR/Cas9-Based Genome Engineering in Human Pluripotent Stem Cells.

Cody Kime1,2,3, Mohammad A Mandegar1,2, Deepak Srivastava1,2,4,5

  • 1Gladstone Institute of Cardiovascular Disease, San Francisco, California.

Current Protocols in Human Genetics
|January 3, 2016
PubMed
Summary
This summary is machine-generated.

CRISPR gene editing offers a simpler, more flexible method for modifying human pluripotent stem cells (hPS cells). This advance accelerates disease modeling and drug discovery using precisely engineered cell lines.

Keywords:
CRISPRCas9genomic engineeringhuman pluripotent stem cells

More Related Videos

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
09:03

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Published on: May 10, 2020

4.7K
CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions
04:21

CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions

Published on: November 1, 2024

1.4K

Related Experiment Videos

Last Updated: Mar 28, 2026

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells
09:04

Generation of Defined Genomic Modifications Using CRISPR-CAS9 in Human Pluripotent Stem Cells

Published on: September 25, 2019

8.9K
Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
09:03

Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

Published on: May 10, 2020

4.7K
CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions
04:21

CRISPR-Cas9 Mediated Gene Deletion in Human Pluripotent Stem Cells Cultured Under Feeder-Free Conditions

Published on: November 1, 2024

1.4K

Area of Science:

  • Stem cell biology
  • Genomics
  • Molecular biology

Background:

  • Human pluripotent stem cells (hPS cells) are crucial for biomedical research, disease modeling, and drug discovery.
  • Previous genome engineering tools like ZFNs and TALENs in hPS cells were complex and limited in adoption.
  • Precise and flexible genome modification is essential for fully utilizing hPS cell potential.

Purpose of the Study:

  • To describe methods for CRISPR endonuclease-mediated genome editing in hPS cells.
  • To enable the creation of hPS cell lines with specific genetic modifications.
  • To facilitate advanced research in disease modeling and developmental studies.

Main Methods:

  • Utilizing clustered regularly interspaced short palindromic repeats (CRISPR) endonuclease technology.
  • Performing genomic editing for insertion/deletion (indel) mutagenesis.
  • Inserting recombinant genomic DNA into hPS cell genomes.

Main Results:

  • Demonstrated the application of CRISPR for efficient genome editing in hPS cells.
  • Enabled the generation of modified hPS cell lines with targeted genetic alterations.
  • Established a simplified and flexible approach compared to older genome engineering techniques.

Conclusions:

  • CRISPR technology significantly simplifies genome engineering in hPS cells.
  • This advancement is expected to accelerate research in various biomedical fields.
  • CRISPR provides a rapid and flexible system for creating genetically modified hPS cell lines for diverse applications.