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Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement

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Published on: January 19, 2017

Nuclear Architecture Organized by Rif1 Underpins the Replication-Timing Program.

Rossana Foti¹, Stefano Gnan¹, Daniela Cornacchia¹

¹Mouse Biology Unit, EMBL Monterotondo, Via Ramarini 32, 00015 Monterotondo, Italy.

|January 4, 2016

Summary

This summary is machine-generated.

Rif1 protein organizes nuclear architecture to control DNA replication timing in mouse cells. This protein links nuclear structure to replication timing, ensuring coordinated DNA synthesis.

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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

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Published on: March 22, 2018

Area of Science:

Molecular Biology
Genetics
Cell Biology

Background:

DNA replication timing is crucial for genome stability but its regulation is not fully understood.
Rif1 protein's role in replication timing regulation is known, but its molecular function remains unclear.

Purpose of the Study:

To elucidate the molecular function of Rif1 in regulating DNA replication timing.
To investigate Rif1's role in nuclear architecture and its connection to replication timing.

Main Methods:

Utilized mouse embryonic stem cells for experiments.
Investigated Rif1 localization and its association with late-replicating domains and Lamin B1.
Assessed the impact of Rif1 loss on replication timing and domain interactions.

Main Results:

Rif1 localizes to late-replicating domains, identifying most of the late-replicating genome with Lamin B1.
Rif1 is essential for replication timing in domains not bound by Lamin B1.
Rif1 regulates interactions between replication-timing domains during G1 phase and coordinates replication during S phase.

Conclusions:

Rif1 acts as a key organizer of nuclear architecture.
Rif1 is the molecular link connecting nuclear architecture to replication-timing establishment in mammals.