Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

22.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
22.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-resolution grayscale printing on Ge thin films via two-beam direct laser writing.

Applied optics·2026
Same author

Stack-based multilayer optical storage devices with sodium silicate as bonding layers.

Applied optics·2025
Same author

A kinematically Bifurcated Metamaterial for Integrated Logic Operation and Computing.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Oxidative stress promotes intervertebral disc degeneration through NAT10-mediated IKKβ N4-acetylcytidine modification.

Cellular signalling·2025
Same author

Impact of Treatment Adjustment on the Endoscopic Prognosis of Postoperative Anastomotic Lesions in Patients With Crohn's Disease.

Clinical and translational gastroenterology·2025
Same author

Predicting Glioma Recurrence Using 18F-FDG PET/CT, MRI, and Tumor Markers: A Combined Approach.

International journal of general medicine·2025
Same journal

Therapeutic potential of crude protein extracts from two Egyptian freshwater snails Lanistes carinatus and Bellamya unicolor.

Scientific reports·2026
Same journal

Microbial contamination of donor corneas and post-keratoplasty endophthalmitis: a comparison between Japanese and U.S. eye banks using cold storage.

Scientific reports·2026
Same journal

Prevalence and contributing factors of virological non-suppression among adult patients on first-line antiretroviral therapy in tertiary hospitals in Ethiopia.

Scientific reports·2026
Same journal

An in vitro comparison of color stability between alkasite and different restorative materials in various staining solutions.

Scientific reports·2026
Same journal

Toward accessible mRNA LNP formulation: systematic evaluation of mixing strategies and key parameters.

Scientific reports·2026
Same journal

A network analysis of personality traits, mentalizing, and psychological health in Chinese college students.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Mar 28, 2026

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
15:06

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

13.5K

Far-field optical imaging with subdiffraction resolution enabled by nonlinear saturation absorption.

Chenliang Ding1,2, Jingsong Wei1

  • 1Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, People's Republic of China.

Scientific Reports
|January 5, 2016
PubMed
Summary
This summary is machine-generated.

Researchers developed a new super-resolution optical imaging method (SAI-SRIM) inspired by SEM. This technique uses nonlinear-saturation-absorption thin films to achieve nanoscale resolution in far-field imaging.

More Related Videos

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.7K
Femtosecond Laser Filaments for Use in Sub-Diffraction-Limited Imaging and Remote Sensing
06:16

Femtosecond Laser Filaments for Use in Sub-Diffraction-Limited Imaging and Remote Sensing

Published on: April 25, 2019

8.1K

Related Experiment Videos

Last Updated: Mar 28, 2026

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle
15:06

Measurement of Scattering Nonlinearities from a Single Plasmonic Nanoparticle

Published on: January 3, 2016

13.5K
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.7K
Femtosecond Laser Filaments for Use in Sub-Diffraction-Limited Imaging and Remote Sensing
06:16

Femtosecond Laser Filaments for Use in Sub-Diffraction-Limited Imaging and Remote Sensing

Published on: April 25, 2019

8.1K

Area of Science:

  • Optics and Photonics
  • Materials Science
  • Nanotechnology

Background:

  • Far-field optical imaging resolution is limited by the Abbe diffraction limit.
  • Conventional methods require specialized equipment or complex procedures for nanoscale imaging.
  • Scanning electron microscopy (SEM) uses conductive coatings to overcome charging effects.

Purpose of the Study:

  • To propose a novel far-field super-resolution optical imaging method (SAI-SRIM).
  • To achieve subdiffraction or nanoscale resolution for imaging sample surfaces.
  • To provide an accessible method for high-resolution surface morphology characterization.

Main Methods:

  • Developed a saturation-absorption-induced far-field super-resolution optical imaging method (SAI-SRIM).
  • Replaced SEM's conductive coatings with nonlinear-saturation-absorption (NSA) thin films.
  • Applied NSA films to sample surfaces using advanced deposition techniques and imaged with laser scanning microscopy.

Main Results:

  • Achieved significantly improved imaging resolution, surpassing the Abbe limit.
  • Obtained subdiffraction-resolved optical images both theoretically and experimentally.
  • Demonstrated effective replication of surface morphologies onto NSA thin films.

Conclusions:

  • SAI-SRIM offers an effective and straightforward approach for far-field super-resolution optical imaging.
  • The method is particularly suitable for samples with complex or fluctuating surface morphologies.
  • This technique enhances optical imaging capabilities for nanoscale surface analysis.