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[Macrophage phagocytosis enhanced by Tiaohengfang polysaccharides in vitro]
Hongfang Zhang1, Penglong Yu2, Yu Zheng2
1Shaanxi University of Traditional Chinese Medicine, Xianyang 712046, China. *Corresponding author,
Objective:
To study the immunoregulatory effect of Tiaohengfang polysaccharides (THFPS) on the phagocytosis of macrophages.
Methods:
According to the preparing method of Chinese medicine polysaccharides, crude polysaccharides were extracted from Tiaohengfang. Mouse peritoneal macrophages were separated and cultured. After the macrophages were treated with (500, 200, 10) μg/mL THFPS for 48 hours, the cell morphology was observed under a light microscope. Phagocytic tests of ink and Staphylococcus aureus were used to evaluate the phagocytosis of the macrophages, and cell enzyme chemical staining was applied to observe the changes of acid phosphatase in macrophages.
Results:
Compared with the control group, the volume of macrophages significantly became bigger when they were treated with (500, 200, 10) μg/mL THFPS, the intake of ink and Staphylococcus aureus significantly increased, and the activity of acid phosphatase was also significantly enhanced, which was positively correlated with the dose of THFPS.
Conclusion:
THFPS can enhance the phagocytosis of macrophages and increase the activity of intracellular acid phosphatase.