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Single CA3 pyramidal cells trigger sharp waves in vitro by exciting interneurones.

Michaël Bazelot¹, Maria T Teleńczuk¹, Richard Miles¹

¹Inserm U1127, CNRS UMR7225, Sorbonne Universités, UPMC Univ Paris 6 UMR S1127, Institut du Cerveau et de la Moelle épinière, Paris, France.

The Journal of Physiology

|January 6, 2016

Summary

This summary is machine-generated.

Single CA3 pyramidal cell firing triggers sharp wave-ripples (SPW-Rs) in the hippocampus by exciting interneurons. This mechanism explains SPW-R initiation but not their repeated firing patterns.

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Area of Science:

Neuroscience
Computational Neuroscience
Systems Neuroscience

Background:

Sharp waves (SPWs) are a form of hippocampal population activity linked to neuronal representations.
The precise synaptic mechanisms underlying SPW generation remain unclear.

Purpose of the Study:

To investigate the synaptic mechanisms responsible for initiating sharp waves (SPWs) in the CA3 hippocampal region.
To determine if single pyramidal cell activity can trigger SPW-like events.

Main Methods:

Using rat hippocampal slices maintained in an interface chamber.
Employing multi-electrode recordings in the CA3 stratum pyramidale.
Stimulating single CA3 pyramidal cells to observe triggered network activity.

Main Results:

Single CA3 pyramidal cell firing reliably triggered SPW-like events at short latencies (2-5 ms).
These triggered events involved putative interneuron spikes followed by field inhibitory postsynaptic potentials (fIPSPs).
Initiated SPWs tended to start near the stimulated cell, unlike spontaneous SPWs which could emerge at multiple sites.

Conclusions:

Pyramidal cell synapses exciting interneurons mediate the initiation of SPWs in vitro.
The study elucidates the initiation mechanism but not the reason for repeated interneuron firing during SPWs.