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Published on: February 1, 2019
Lipoplexes of dicationic gemini surfactants with DNA: Structural features of DNA compaction and transfection
D A Faizullin1, Yu F Zuev2, L Ya Zakharova3
1Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Center of the Russian Academy of Sciences, Kazan, Russia. dfaizullin@mail.ru.
Abstract:
The internal structure of DNA lipoplexes with hydroxyethylated alkylammonium gemini surfactants (GS) with high transfection activity was studied by circular dichroism. It was shown that the efficiency of transfection of HEK293T cells with the pEGFP-N1 circular plasmid was different from zero only in the region of existence of chiral supramolecular DNA-GS complexes and reaches a maximum at concentrations at which the spontaneous aggregation of components is observed.
