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Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
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PML isoform II plays a critical role in nuclear lipid droplet formation

Yuki Ohsaki1, Takeshi Kawai1, Yukichika Yoshikawa1

  • 1Department of Anatomy and Molecular Cell Biology, Nagoya University Graduate School of Medicine, Nagoya 466-8550, Japan.

The Journal of Cell Biology
|January 6, 2016
PubMed

Insights

Premyeloctyic leukemia (PML) isoform II is crucial for generating nuclear lipid droplets (LDs) in specific cells. This protein interacts with nuclear bodies and the inner nuclear membrane, influencing LD formation.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Lipid Metabolism

Background:

  • Lipid droplets (LDs) are observed within the nucleus of certain cell types.
  • These nuclear LDs are associated with premyelocytic leukemia (PML) nuclear bodies (NBs) and type I nucleoplasmic reticulum (NR).

Purpose of the Study:

  • To investigate the role of PML isoform II (PML-II) in the formation and regulation of nuclear LDs.
  • To elucidate the relationship between PML-II, nuclear LDs, and the nuclear envelope.

Main Methods:

  • Utilized knockdown and overexpression techniques for PML-II in hepatocyte-derived cell lines.
  • Investigated the impact of SUN protein knockdown on nuclear LDs and type I NR.
  • Analyzed the protein content and lipid ester incorporation within nuclear LDs.

Main Results:

  • PML-II knockdown decreased nuclear LDs and type I NR; overexpression increased them in specific cell types.
  • PML-II localized to PML NBs and the nuclear envelope, excluding lamins and SUN proteins.
  • SUN protein knockdown increased type I NR and nuclear LDs, an effect reversed by PML-II knockdown.
  • Nuclear LDs contain diacylglycerol O-acyltransferase 2 and CTP:phosphocholine cytidylyltransferase α, and incorporate newly synthesized lipid esters.

Conclusions:

  • PML-II plays a critical role in the generation of nuclear LDs in specific cell types.
  • PML-II's interaction with nuclear structures is key to its function in nuclear LD formation.

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