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Related Experiment Video

Updated: Mar 28, 2026

Split-Ubiquitin Based Membrane Yeast Two-Hybrid MYTH System: A Powerful Tool For Identifying Protein-Protein Interactions

Split-Ubiquitin Based Membrane Yeast Two-Hybrid MYTH System: A Powerful Tool For Identifying Protein-Protein Interactions

Published on: February 1, 2010

MYTH Screening: iMYTH and tMYTH Variants.

Jamie Snider¹, Igor Stagljar²

¹Donnelly Centre, University of Toronto, Toronto, Ontario M5S 3E1, Canada;

Cold Spring Harbor Protocols

|January 6, 2016

Summary

This summary is machine-generated.

High-throughput screening using the membrane yeast two-hybrid (MYTH) assay allows for rapid generation of detailed protein interaction maps. This method, applicable to validated baits, can be completed in approximately 3-4 weeks.

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Split-Ubiquitin Based Membrane Yeast Two-Hybrid MYTH System: A Powerful Tool For Identifying Protein-Protein Interactions

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Published on: February 1, 2010

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA

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Published on: November 23, 2019

Area of Science:

Molecular Biology
Biochemistry
Genomics

Background:

The membrane yeast two-hybrid (MYTH) assay is a powerful tool for studying protein-protein interactions.
Validated MYTH baits are crucial for initiating interaction studies.

Purpose of the Study:

To describe the methodology for conducting high-throughput MYTH library screening.
To provide a protocol for generating comprehensive protein interaction maps (interactomes).

Main Methods:

Utilizing a validated bait in the MYTH assay for library screening.
Implementing high-throughput screening protocols for MYTH assays.
Adaptation of principles for smaller-scale interaction studies.

Main Results:

Successful high-throughput screening of MYTH libraries is achievable.
Detailed interaction maps can be generated efficiently.

Conclusions:

High-throughput MYTH screening is an effective strategy for interactome mapping.
The described MYTH screening protocol is adaptable and efficient, with completion in approximately 3-4 weeks.