Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

17.7K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
17.7K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.8K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.8K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

995
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
995

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

activeDISCO enables large-scale exploration of human brain anatomy by light sheet microscopy.

Communications biology·2026
Same author

Can Machines Identify Pain Effects? A Machine Learning Proof of Concept to Identify EMG Pain Signature.

Bioengineering (Basel, Switzerland)·2026
Same author

NPY2R Agonist-Induced Gastric Effects Leading to Intestinal Dysbiosis and Secondary Intestinal Pathology in CD1 Mice.

Toxicologic pathology·2026
Same author

Zebrafish genetic model of neuromuscular degeneration associated with Atrogin-1 expression.

PLoS genetics·2026
Same author

Disrupted Corticomuscular Coherence and Force Steadiness During Acute Low Back Pain.

Bioengineering (Basel, Switzerland)·2025
Same author

Beyond Low Back Pain! The Influence of Physical Activity on Mental Health, Reflected in the Functionality of People with Low Back Pain.

Healthcare (Basel, Switzerland)·2025
Same journal

<i>Neurophotonics</i> book club: "The Secret of Secrets" by Dan Brown - a thought-provoking twist on a famous neuroscience controversy.

Neurophotonics·2026
Same journal

Measurement of the absolute value of the optical birefringence of myelin in primate brain tissue.

Neurophotonics·2026
Same journal

Data-driven optimization of preschoolers' hemodynamic response in a VR setup: advancing analytic methods for children's fNIRS naturalistic data with the AICopt method.

Neurophotonics·2026
Same journal

Multimodal optical imaging combining voltage-sensitive dye ElectroFluor630 with genetically encoded calcium, glutamate, or voltage indicators.

Neurophotonics·2026
Same journal

Data-driven wavelet coherence approach to assess neurovascular coupling in neonatal hypoxic-ischemic encephalopathy.

Neurophotonics·2026
Same journal

Effect of sleep stage on patterns of fNIRS hemodynamic response to auditory paradigms in 1-month-old Gambian and UK infants.

Neurophotonics·2026
See all related articles

Related Experiment Video

Updated: Mar 28, 2026

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging
07:14

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging

Published on: April 11, 2025

1.4K

Ultramicroscopy: development and outlook.

Hans-Ulrich Dodt1, Saiedeh Saghafi1, Klaus Becker1

  • 1Vienna University of Technology, FKE, Chair of Bioelectronics, Floragasse 7, 1040 Vienna, Austria; Medical University of Vienna, Center for Brain Research, Spitalgasse 4, 1090 Vienna, Austria.

Neurophotonics
|January 6, 2016
PubMed
Summary
This summary is machine-generated.

We developed ultramicroscopy to image whole mouse brains and embryos in 3-D. This technique preserves fluorescence and achieves high resolution, advancing biological and medical imaging.

Keywords:
brain clearinglight sheetobjective correctionthree-dimensional brain imagingtumor clearingultramicroscopy

More Related Videos

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

12.1K
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.7K

Related Experiment Videos

Last Updated: Mar 28, 2026

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging
07:14

Microfabrication of Implantable Optics Integrated in a Microstructured Imaging Window for Advanced In Vivo Imaging

Published on: April 11, 2025

1.4K
Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
08:41

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Published on: August 16, 2012

12.1K
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.7K

Area of Science:

  • Biomedical Imaging
  • Microscopy Techniques
  • Neuroscience

Background:

  • Ultramicroscopy builds upon 100 years of microscopy advancements.
  • Previous methods lacked the resolution and depth for whole-organ imaging.

Purpose of the Study:

  • To present a developed ultramicroscopy technique for high-resolution 3-D imaging.
  • To demonstrate its application in imaging entire mouse brains, embryos, and human tissues.

Main Methods:

  • Developed a light sheet microscope and a chemical clearing method (3DISCO).
  • Optimized light sheet generation using aspheric optics for improved optical sectioning.
  • Corrected air microscope objectives for high refractive index clearing solutions.

Main Results:

  • Successfully imaged GFP-labeled neurons in whole mouse brains with preserved fluorescence.
  • Achieved high-resolution 3-D recordings of mouse embryos, flies, and human breast cancer tissue.
  • Demonstrated potential for super-resolution via stimulated emission depletion (STED) microscopy.

Conclusions:

  • The developed ultramicroscopy technique enables high-resolution 3-D imaging of large biological samples.
  • This method has broad applications in neuroscience, developmental biology, and medical diagnostics.
  • Future work includes integrating super-resolution capabilities for enhanced detail.