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The expression of enterotoxin A-B+ gene of V. cholerae in E. coli

Q J Ma¹, J G Zhou, X Q Yu

¹Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing.

Science in China. Series B, Chemistry, Life Sciences & Earth Sciences

|February 1, 1989

Abstract:

The cloning in E. coli of a cholerae toxin gene that is A-B+ has been successfully constructed by using DNA recombinant techniques. E. coli cells carrying the recombinant plasmid pMM-CTB have been shown to produce a large amount of CTB subunits which are secreted as extracellular proteins.

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