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Area of Science:

  • Plasma physics
  • Microbiology
  • Biophysics

Background:

  • Controlling bacterial contamination is crucial in various fields.
  • Microplasma jets offer a novel approach for sterilization.
  • Understanding the inactivation mechanisms is essential for optimizing applications.

Purpose of the Study:

  • To investigate the inactivation efficacy of a self-made microplasma jet system against Escherichia coli.
  • To identify the key parameters influencing the antimicrobial effect.
  • To elucidate the inactivation mechanisms at the cellular level.

Main Methods:

  • Utilized a custom-built microplasma jet system for bacterial inactivation experiments.
  • Quantified inactivation using logarithmic concentration and antimicrobial ring size.
  • Systematically varied parameters like airflow rate, treatment distance, and carrier gas composition.
  • Analyzed the contribution of different microplasma components (active species, heat, UV, charged particles).
  • Examined cellular changes using scanning electron microscopy (SEM).

Main Results:

  • Achieved significant inactivation of Escherichia coli within 30 seconds.
  • Optimal inactivation observed with oxygen as the carrier gas.
  • Inactivation efficacy was dependent on airflow rate and treatment distance.
  • Electrically neutral active species were identified as the primary inactivation factor.
  • Microplasma demonstrated an etching effect on the cell membrane and degraded proteins.
  • SEM revealed progressive changes in bacterial cell morphology.

Conclusions:

  • Microplasma jets are highly effective for rapid bacterial inactivation.
  • Electrically neutral active species are the main drivers of antimicrobial activity.
  • Microplasma exerts physical and chemical effects on bacterial cells, leading to inactivation.
  • This technology holds promise for sterilization and disinfection applications.