Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Assessment of phagocytic function using chemiluminescence.

H S Hosker1, C Kelly, P A Corris

  • 1Department of Respiratory Medicine, Freeman Hospital, Newcastle upon Tyne, UK.

Blood Reviews
|June 1, 1989
PubMed
Summary

Cellular chemiluminescence offers a reliable way to measure phagocytic function by detecting light produced during the respiratory burst. This method accurately assesses immune cell activity in response to various factors.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Strengthening incident learning in radiotherapy practice: insights from the MARLIN study.

Clinical and translational radiation oncology·2026
Same author

Authors' Response to Comment on "High Incidence of Mandibular Osteoradionecrosis After Radiotherapy for Oral Cavity: Time for Rebalancing Risks and Benefits".

Clinical oncology (Royal College of Radiologists (Great Britain))·2026
Same author

Association of patient factors and health-related quality of life with weight changes during chemotherapy for colorectal cancer: secondary analysis of the SCOT trial.

Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2026
Same author

High Incidence of Mandibular Osteoradionecrosis After Radiotherapy for Oral Cavity: Time for Rebalancing Risks and Benefits.

Clinical oncology (Royal College of Radiologists (Great Britain))·2026
Same author

Impact of COVID-19 and factors associated with long COVID and COVID-19 vaccine uptake in people with HIV in the United Kingdom: Results from Positive Voices 2022.

HIV medicine·2025
Same author

Genomic characterization of <i>Pseudomonas aeruginosa</i> from canine otitis highlights the need for a One Health approach to this opportunistic pathogen.

Microbial genomics·2025

Area of Science:

  • Immunology
  • Biochemistry

Background:

  • Phagocytic cells are crucial for host defense.
  • Their bactericidal activity relies on the respiratory burst, producing reactive oxygen species.
  • Chemiluminescence quantifies this process via light emission.

Purpose of the Study:

  • To explain the theory and application of cellular chemiluminescence.
  • To detail methods for assessing phagocytic function.
  • To discuss clinical relevance.

Main Methods:

  • Utilizing chemiluminescent probes to amplify light emission from reactive oxygen species.
  • Measuring light output using a luminometer.
  • Stimulating phagocytic cells (polymorphonuclear leukocytes, monocytes, macrophages) with various agents.

Related Experiment Videos

Main Results:

  • Demonstrated a close correlation between chemiluminescence and other phagocytic function assessments.
  • Showcased the technique's ability to evaluate immune cell responses.
  • Highlighted its utility in assessing effects of disease, drugs, and toxins.

Conclusions:

  • Cellular chemiluminescence is a simple, reliable method for assessing phagocytic function.
  • The technique is versatile for various immune cells and applications.
  • It provides valuable insights into immune responses and potential therapeutic interventions.