Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Hand hygiene01:23

Hand hygiene

6.5K
Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
Hand washing...
6.5K
Skin Diseases and Disorders01:23

Skin Diseases and Disorders

6.1K
Skin is the first line of defense and encounters a variety of microbes. Some pathogenic strains are often the cause of a broad range of infections of the skin and other body systems. These conditions can affect people of all ages and may have different causes, including genetic factors, infections, autoimmune reactions, environmental factors, and lifestyle choices.
Gram-positive Staphylococcus spp. and Streptococcus spp. are responsible for many of the most common skin infections. However, many...
6.1K
The Skin Microbiota01:27

The Skin Microbiota

39
The human skin serves as a complex ecosystem inhabited by a diverse community of microorganisms, including bacteria, fungi, and viruses. This microbiome plays a critical role in maintaining skin health and defending against pathogenic invaders. The composition of microbial communities varies significantly across different regions of the body, influenced primarily by the local levels of moisture and sebum.Regional Variation in Skin MicrobiotaCutibacterium acnes predominantly colonizes sebaceous...
39
Drug Toxicity: Allergic Reactions01:30

Drug Toxicity: Allergic Reactions

132
Drug-related allergies are immune-mediated responses triggered by the administration of pharmacological agents. These hypersensitivity reactions are classified based on the immune mechanisms involved. The four primary types—Type I, II, III, and IV—are mediated by different immunological pathways and exhibit distinct clinical manifestations.Type I Hypersensitivity/ IgE-Mediated Reactions: Immunoglobulin E (IgE) immediately mediates Type I hypersensitivity reactions. Upon initial...
132
Staphylococcal Skin Infections01:29

Staphylococcal Skin Infections

46
Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...
46

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A multicenter study by the Brazilian Society of Dermatology on a new standard patch test series.

Anais brasileiros de dermatologia·2026
Same author

Diagnostic Performance of the Trichogram Compared to Histopathology of Scalp Biopsy in Horizontal Sections in Female Androgenetic Alopecia: A Retrospective Study.

Skin appendage disorders·2026
Same author

Hair Transplant in Patchy Scarring Alopecia of the Fronto-Temporal Area.

Skin appendage disorders·2025
Same author

Chronic actinic dermatitis: a retrospective study of epicutaneous and photo epicutaneous tests between 2007‒2023.

Anais brasileiros de dermatologia·2024
Same author

Evaluation of Axillary Skin Thickness by High-Frequency Ultrasound.

Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.]·2024
Same author

Normal Trichoscopic Findings after Hair Transplantation in Androgenetic Alopecia: A Prospective Case Series.

Skin appendage disorders·2023

Related Experiment Video

Updated: Mar 27, 2026

An In Vitro Skin Irritation Test SIT using the EpiDerm Reconstructed Human Epidermal RHE Model
21:16

An In Vitro Skin Irritation Test SIT using the EpiDerm Reconstructed Human Epidermal RHE Model

Published on: July 13, 2009

69.3K

Contact dermatitis to methylisothiazolinone.

Maria Antonieta Rios Scherrer1, Vanessa Barreto Rocha1, Ana Regina Coelho Andrade1

  • 1Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.

Anais Brasileiros De Dermatologia
|January 7, 2016
PubMed
Summary
This summary is machine-generated.

Increasing cases of contact dermatitis are linked to Methylisothiazolinone (MI) preservative. Testing with MI 2000 ppm, not in standard trays, identified reactions in patients, highlighting its importance in diagnosis.

More Related Videos

A Mouse Ear Model for Allergic Contact Dermatitis Evaluation
08:02

A Mouse Ear Model for Allergic Contact Dermatitis Evaluation

Published on: March 24, 2023

5.3K
Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis
08:25

Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis

Published on: September 26, 2022

3.7K

Related Experiment Videos

Last Updated: Mar 27, 2026

An In Vitro Skin Irritation Test SIT using the EpiDerm Reconstructed Human Epidermal RHE Model
21:16

An In Vitro Skin Irritation Test SIT using the EpiDerm Reconstructed Human Epidermal RHE Model

Published on: July 13, 2009

69.3K
A Mouse Ear Model for Allergic Contact Dermatitis Evaluation
08:02

A Mouse Ear Model for Allergic Contact Dermatitis Evaluation

Published on: March 24, 2023

5.3K
Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis
08:25

Contact Hypersensitivity as a Murine Model of Allergic Contact Dermatitis

Published on: September 26, 2022

3.7K

Area of Science:

  • Dermatology
  • Toxicology
  • Allergen Testing

Background:

  • Methylisothiazolinone (MI) is a widely used preservative in cosmetics and industrial products.
  • There is a documented rise in contact dermatitis cases attributed to Methylchloroisothiazolinone/Methylisothiazolinone (MCI/MI) and MI.
  • Standard patch testing protocols may not adequately identify MI-specific allergies.

Purpose of the Study:

  • To evaluate the diagnostic utility of epicutaneous testing with aqueous MI 2000 ppm.
  • To assess the frequency of MI-induced contact dermatitis in a Brazilian patient cohort.
  • To highlight cases where MI allergy is present despite a negative reaction to MCI/MI.

Main Methods:

  • A series of 23 patients were subjected to epicutaneous testing.
  • Testing included the Brazilian standard tray and aqueous MI 2000 ppm.
  • Patient reactions to both MI and MCI/MI were recorded and analyzed.

Main Results:

  • The study identified contact dermatitis reactions to MI in the tested patient group.
  • A specific case demonstrated a negative reaction to MCI/MI but a positive reaction to MI.
  • The findings underscore the need for specific MI testing.

Conclusions:

  • Epicutaneous testing with aqueous MI 2000 ppm is crucial for diagnosing MI-related contact dermatitis.
  • The inclusion of MI testing in standard dermatological assessments is recommended.
  • MI allergy can occur independently of MCI/MI sensitivity.