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In Vitro Thrombosis Test for Ventricular Assist Devices
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A Method for Creating Artificial Thrombi In Vitro Using a Rotating Mechanical Surface.

Staci L Jessen1, Andrew M Masse, Mallory D Carpenter

  • 1From the *Department of Biomedical Engineering, †College of Veterinary Medicine, and ‡Department of Veterinary Pathobiology, Texas A&M University, College Station, Texas.

ASAIO Journal (American Society for Artificial Internal Organs : 1992)
|January 7, 2016
PubMed
Summary
This summary is machine-generated.

Researchers developed a method to create artificial fibrin clots for ventricular assist device (VAD) testing. This technique simulates thromboembolism in vitro, aiding VAD response studies.

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Area of Science:

  • Biomedical Engineering
  • Cardiovascular Research
  • Materials Science

Background:

  • Thromboembolism is a significant risk in ventricular assist device (VAD) therapy.
  • Understanding VAD response to thromboembolism requires controlled in vitro testing.
  • Monitoring pump parameters during thromboembolism exposure is crucial.

Purpose of the Study:

  • To develop a reproducible method for creating standardized fibrin thrombi.
  • To enable in vitro testing of VAD response to thromboembolism.
  • To generate artificial clots mimicking clinical VAD thrombi.

Main Methods:

  • Donor equine blood was collected using sodium citrate.
  • Calcium chloride (CaCl2) was added to induce clotting.
  • Shear forces were applied using a rotating component at varying speeds and distances.

Main Results:

  • The method produced standardized fibrin clots.
  • Artificial clots exhibited microscopic features similar to clinical VAD thrombi.
  • Higher rotational speeds and smaller clearances yielded clots resembling in vivo thrombi.

Conclusions:

  • This method effectively creates artificial fibrin clots for VAD thromboembolism studies.
  • The standardized clots allow for controlled in vitro assessment of VAD performance.
  • This approach supports research into mitigating VAD-related thromboembolic complications.