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Related Experiment Videos

Image based quantitative reader for Lateral flow immunofluorescence assay.

Kaushik Basak Chowdhury, Jayaraj Joseph, Mohanasankar Sivaprakasam

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
    |January 7, 2016
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    Immunofluorescence Microscopy01:12

    Immunofluorescence Microscopy

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    A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
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    A new image-based reader offers accurate, automated quantitative analysis for fluorescence lateral flow immunoassays (LFIA). This reliable system enhances point-of-care testing (POCT) diagnostics for various applications.

    Area of Science:

    • Biomedical Engineering
    • Analytical Chemistry
    • Medical Diagnostics

    Background:

    • Fluorescence Lateral Flow Immunoassays (LFIA) are crucial for Point-of-Care Testing (POCT).
    • Accurate, automated quantitative readers are essential for reliable LFIA results in POCT.
    • Existing methods may lack the integration and precision required for widespread diagnostic use.

    Purpose of the Study:

    • To develop and validate an integrated, motion-free, image-based reader for automated quantitative analysis of LFIA test strips.
    • To demonstrate the accuracy and reliability of the developed reader for POCT applications.
    • To assess the reproducibility of the system using reference and clinical test cartridges.

    Main Methods:

    • An image-based quantitative method utilizing a line laser diode for illumination of LFIA test strips with fluorescent dye.

    Related Experiment Videos

  • Employing an emission filter to capture fluorescence light from the region of interest (ROI).
  • Automated image processing and analysis via a dedicated control program for quantitative results.

    • Main Results:

    • Successful development of an automated reader for quantitative LFIA analysis.
    • Demonstrated reproducibility using reference and Glycated Haemoglobin (HbA1c) test cartridges.
    • The system provides accurate and reliable quantitative measurements from LFIA test strips.

    Conclusions:

    • The developed image-based reader provides an accurate and automated solution for LFIA quantification.
    • This system enhances the capabilities of point-of-care testing (POCT) diagnostics.
    • The proposed reader is adaptable for compact design, enabling widespread adoption in LFIA testing.