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Transparency of nitrocellulose membranes with Triton X-114
1Department of Tropical Public Health, Harvard School of Public Health, Boston, MA 02115.
Electrophoresis
|July 1, 1989
Abstract:
Transparency of nitrocellulose membranes can be realized by simply impregnating the filters with concentrated Triton X-114. Within a few seconds, protein blots become transparent and can be photographed by transillumination or scanned on a densitometer. Removal of the detergent is possible and the recovered filters can further be used for immunodetection purposes. This procedure is particularly wellsuited where extensive investigation of a single separated protein sample is required.