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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

100.9K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
100.9K
RNA-seq03:21

RNA-seq

12.5K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.5K
Sanger Sequencing01:57

Sanger Sequencing

778.3K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
778.3K
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

13.6K
In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
13.6K
Comparing Copy Number Variations and SNPs02:26

Comparing Copy Number Variations and SNPs

19.2K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
19.2K

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Related Experiment Video

Updated: Mar 27, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

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Performance comparison of Next Generation sequencing platforms.

Bekir Erguner, Duran Ustek, Mahmut S Sagiroglu

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference
    |January 7, 2016
    PubMed
    Summary

    Next Generation DNA Sequencing (NGS) platforms provide high throughput at low cost, driving clinical adoption. This study evaluates the sequencing accuracy of three leading NGS technologies: Ion Torrent, Roche GS FLX+, and Illumina HiSeq 2000.

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    Area of Science:

    • Genomics and Molecular Biology
    • Biotechnology

    Background:

    • Next Generation DNA Sequencing (NGS) technologies have revolutionized biological research and clinical diagnostics.
    • Increasing adoption by clinicians necessitates a thorough understanding of platform performance.
    • Key concerns include sequencing throughput, cost-effectiveness, and accuracy.

    Purpose of the Study:

    • To compare the sequencing accuracy of three prominent Next Generation DNA Sequencing platforms.
    • To provide data to researchers and clinicians for informed platform selection.

    Main Methods:

    • Comparative analysis of sequencing data generated from three widely used NGS platforms.
    • Platforms evaluated include Ion Torrent (Life Technologies), GS FLX+ (Roche), and HiSeq 2000 (Illumina).

    Main Results:

    • Performance metrics related to sequencing accuracy were assessed for each platform.
    • Comparative accuracy data across the three platforms were generated.

    Conclusions:

    • Sequencing accuracy is a critical factor for researchers and clinicians adopting NGS technologies.
    • Understanding the comparative accuracy of different NGS platforms is essential for reliable genomic applications.