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Related Experiment Videos

Post-translational modifications in mitotic yeast cells.

L T Nevalainen1, J Louhelainen, M Makarow

  • 1Institute of Biotechnology, University of Helsinki, Finland.

European Journal of Biochemistry
|September 1, 1989
PubMed
Summary

Yeast secretion continues during mitosis, unlike in mammalian cells. The yeast secretory pathway remains fully functional throughout the cell cycle, processing glycoproteins and pheromones.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Yeast Genetics

Background:

  • Secretion in Saccharomyces cerevisiae continues during mitosis, contrasting with mammalian cells where membrane traffic halts.
  • Previous work demonstrated uninterrupted invertase secretion in yeast during mitosis.

Purpose of the Study:

  • To investigate the functionality of the yeast secretory pathway during mitosis.
  • To determine if cell surface glycoproteins, mannans, and alpha-factor traverse the secretory pathway during mitosis.
  • To assess the capacity of mitotic yeast cells for post-translational modification of secretory proteins.

Main Methods:

  • Analysis of invertase secretion and post-translational modifications in mitotic yeast.
  • Tracking of bulk cell surface glycoproteins and mannans through the secretory pathway.

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  • Examination of alpha-factor processing and secretion during mitosis.

    • Main Results:

    • Bulk cell surface glycoproteins and mannans, along with alpha-factor, are transported through the secretory pathway during mitosis.
    • Mitotic yeast cells perform various post-translational modifications: invertase oligomerization and glycosylation, N-glycan core extension, N-glycan phosphorylation, O-glycan extension, and alpha-factor proteolytic processing.
    • These findings indicate a fully functional secretory pathway in yeast throughout the cell cycle.

    Conclusions:

    • The yeast secretory pathway is fully operational during mitosis.
    • Cell cycle progression does not impede essential secretory functions in Saccharomyces cerevisiae.
    • Yeast provides a model for studying continuous secretion in dividing cells.