High-throughput analysis of tissue-based biomarkers in digital pathology

By simultaneously processing a large number of tissue samples, the tissue microarray (TMA) technology allows standardized screening of protein expression using immunohistochemistry thereby providing a very efficient way for tissue-based biomarker analysis. Nowadays, whole slide imaging is becoming standard in digital pathology and enables image sharing, archiving and also processing. In this paper, we present methods for processing TMA images in order to correctly identify the numerous tissue samples and to register images from consecutive TMA sections.