[Effects of resveratrol on Nrf2 signal pathway of chronic lead-exposed mouse brain tissue]

To evaluate the effect of resveratrol on NF-E2-related factor 2 (Nrf2) signal pathway in the process of oxidative stress of mice brain tissue induced by chronic lead exposure.

A total of 48 healthy weaned C57BL/6 male mice were randomly divided into four groups (12 mice each group), control group, lead poisoning model group, lead poisoning coupled with resveratrol intervention group and resveratrol group. The lead poisoning mice model had been made by exposed to 0.2% lead acetate solution in the drinking water for 12 weeks. At the same time, the mice in the intervention group and resveratrol group were fed with resveratrol (50 mg/(kg · d)) by oral gavage and the other two groups were treated with the same volume of solvent, sodium carboxymethylcellulose. The serum and brain tissues were removed and used for detecting the lead concentration and measuring the activity of GSH-Px and the content of GSH and MDA. The levels of protein Nrf2 and γ-GCS were determined by western blot.

Compared with the poisoning model group, GSH-Px activity and GSH content were significantly increased (P < 0.05), and MDA content was significantly decreased in the brain tissue intervened by resveratrol (P < 0.05). The protein expression of Nrf2 and γ-GCS were induced in the brain tissue of the intervention group (P < 0.05).

Resveratrol could reduce the oxidation damage caused by chronic lead exposure in drinking water which may due to the protein activation of Nrf2, further up-regulated expression of targeting γ-GCS and adjusted dynamic balance of GSH system.