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Updated: Mar 27, 2026

Preparation of Expanded Chitin Foams and their Use in the Removal of Aqueous Copper
Published on: February 27, 2021
Optimization of medium components for production of chitin deacetylase by Bacillus amyloliquefaciens Z7, using
Yuanhao He1, Jianping Xu1, Shengjie Wang1
1Key Laboratory for Non-wood Forest Cultivation and Conservation of the Ministry of Education, Central South University of Forestry and Technology, Changsha, P.R. China; College of Forestry, Central South University of Forestry and Technology, Changsha, P.R. China.
Abstract:
Plackett-Burman design and Box-Behnken response surface methodology (RSM) was employed to optimize the medium components for the chitin deacetylase (CDA) activity from Bacillus amyloliquefaciens Z7. Plackett-Burman design was applied to determine the specific medium components affecting CDA activity and found that starch, chitin and MgSO4 were critical in augmenting CDA activity. These significant parameters were further optimized using Box-Behnken RSM and the optimum concentrations of starch, chitin and MgSO4 were found to be 24.4, 8.8 and 0.19 g/L, respectively. The optimum medium composition was chitin 8.8 g/L, starch 24.4 g/L, yeast extract 10g/L, MgSO4 0.19 g/L, K2HPO4 0.3 g/L and NaCl 5 g/L. Under these optimal conditions, the CDA activity of Bacillus amyloliquefaciens Z7 increased distinctly from 18.75 to 27.48 U/mL (46.6% increase in total yield).
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