Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Immunoprecipitation01:20

Immunoprecipitation

7.9K
Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
7.9K
Chromatin Immunoprecipitation- ChIP02:36

Chromatin Immunoprecipitation- ChIP

12.8K
Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...
12.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Play stimulation modifies brain and behaviour in chickens - effects of domestication.

Behavioural brain research·2026
Same author

Benchmarking of methods to analyse data derived from GBS-MeDIP.

BMC bioinformatics·2026
Same author

Individual variation in play in young chickens - assessment and connection to affective state and personality.

Scientific reports·2026
Same author

Transgenerational Consequences of Imidacloprid Larval Diet Contamination in the Sheep Blowfly <i>Lucilia sericata</i> (Diptera: Calliphoridae).

Insects·2025
Same author

[Christmas article: Flaming heart syndrome: Shared false memories in Danish popular culture].

Ugeskrift for laeger·2025
Same author

Epigenetic changes and their potential reversibility in mental health disorders.

Essays in biochemistry·2025
Same journal

Facile synthesis of model polystyrene nanoparticles for nanoplastics research.

MethodsX·2026
Same journal

Effectiveness of a posture education program in high school students: A randomized controlled trial protocol.

MethodsX·2026
Same journal

Development and characterization of silicone-based testosterone propionate implants for sustained androgen delivery in juvenile castrated male pigs.

MethodsX·2026
Same journal

Machine learning assisted multi-criteria decision-making approaches for site selection: A systematic review.

MethodsX·2026
Same journal

A systematic analytical framework for multi-source municipal solid waste characterization for energy recovery.

MethodsX·2026
Same journal

Decision tree and reinforcement learning for contextual electricity consumption forecasting in buildings.

MethodsX·2026
See all related articles

Related Experiment Video

Updated: Mar 27, 2026

Methylated DNA Immunoprecipitation
21:24

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

24.4K

Optimized method for methylated DNA immuno-precipitation.

Carlos Guerrero-Bosagna1, Per Jensen1

  • 1Avian Behavioral Genomics and Physiology Group, IFM Biology, Linköping University, Linköping 58 183, Sweden.

Methodsx
|January 8, 2016
PubMed
Summary
This summary is machine-generated.

Optimizing Methylated DNA Immunoprecipitation (MeDIP) significantly reduces protocol time by half. This streamlined DNA methylation analysis method maintains result quality for faster genomic insights.

Keywords:
DNA methylationEpigeneticImmunoprecipitationMeDIPWhole genome

More Related Videos

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
14:29

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Published on: May 1, 2013

14.8K
Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

8.0K

Related Experiment Videos

Last Updated: Mar 27, 2026

Methylated DNA Immunoprecipitation
21:24

Methylated DNA Immunoprecipitation

Published on: January 2, 2009

24.4K
Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass
14:29

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Published on: May 1, 2013

14.8K
Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

8.0K

Area of Science:

  • Molecular Biology
  • Genomics
  • Epigenetics

Background:

  • Methylated DNA Immunoprecipitation (MeDIP) is crucial for whole-genome DNA methylation analysis.
  • MeDIP utilizes antibodies specific to methyl-cytosine to capture methylated DNA fractions.
  • Previous MeDIP protocols were time-consuming, often taking around 3 days.

Purpose of the Study:

  • To optimize the Methylated DNA Immunoprecipitation (MeDIP) protocol.
  • To significantly reduce the overall procedure time without compromising data quality.
  • To enhance the efficiency of DNA methylation analysis.

Main Methods:

  • Reduced the number of washes at various protocol stages after careful evaluation.
  • Shortened reaction times for detaching methylated DNA from beads and antibodies.
  • Modified DNA purification methods, incorporating new devices and eliminating phenol-chloroform extraction.

Main Results:

  • The optimized MeDIP protocol length was reduced by 50%.
  • The quality of results from the optimized protocol remained comparable to the original method.
  • The streamlined protocol provides faster turnarounds for DNA methylation studies.

Conclusions:

  • The optimized MeDIP protocol offers a significantly faster and efficient approach to whole-genome DNA methylation analysis.
  • This optimization makes MeDIP more accessible for time-sensitive research.
  • The modified protocol maintains high-quality results, crucial for accurate epigenetic studies.