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Updated: Mar 27, 2026
Continuous Fluorescence-Based Endonuclease-Coupled DNA Methylation Assay to Screen for DNA Methyltransferase Inhibitors
Published on: August 5, 2022
Target-protected dumbbell molecular probe mediated cascade rolling circle amplification strategy for the sensitive
Haiyan Zhao1, Lei Wang, Wei Jiang
1Key Laboratory for Colloid and Interface Chemistry of Education Ministry, School of Chemistry and Chemical Engineering, Shandong University, 250100 Jinan, P. R. China. wjiang@sdu.edu.cn.
Abstract:
A novel fluorescence detection system was developed for DNA methyltransferase (MTase) activity assay based on a target-protected dumbbell molecular probe mediated cascade rolling circle amplification. It showed excellent specificity and sensitivity with a detection limit of 0.0024 U mL(-1), and potential application in quantitatively monitoring MTase activity and screening of anticancer drugs.
