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Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
09:01

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Published on: March 26, 2018

The transmural migration and release of blood cells in acute myelogenous leukemia

P P Bruyn, R P Becker, S Michelson

    The American Journal of Anatomy
    |June 1, 1977
    PubMed

    Insights

    Malignant cells in myelogenous leukemia create temporary pores to enter bone marrow sinusoids, even as the vessel lining remains intact. This study reveals unique cellular migration and vessel changes in leukemia progression.

    Area of Science:

    • Hematology
    • Oncology
    • Cell Biology

    Background:

    • Myelogenous leukemia is characterized by increased bone marrow cellularity and circulating myeloid cells.
    • Understanding malignant cell transmural passage is crucial for leukemia research.
    • The Shay myelogenous leukemia rat model offers insights into disease progression.

    Purpose of the Study:

    • To investigate the transmural passage of malignant blood cells into bone marrow sinusoids.
    • To examine the integrity of the sinusoidal endothelial lining during leukemia.
    • To identify mechanisms of malignant cell entry and associated vascular changes.

    Main Methods:

    • Light microscopy
    • Transmission electron microscopy (TEM)
    • Scanning electron microscopy (SEM)
    • Shay myelogenous leukemia rat model

    Main Results:

    • The sinusoidal endothelial lining remains intact and continuous, even in advanced leukemia.
    • Malignant myeloblast-like cells utilize temporary migration pores for sinusoidal entry.
    • Nondegenerative changes include altered sinusoid patterns and intraluminal cytoplasmic bridges formed by endothelial cells.

    Conclusions:

    • Malignant cell transmural passage in myelogenous leukemia occurs via transient pores without compromising endothelial integrity.
    • Endothelial cells undergo adaptive changes, forming structures that may impede blood flow.
    • These findings provide a detailed ultrastructural view of leukemia cell trafficking in the bone marrow.

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