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A self-setting iPSMSC-alginate-calcium phosphate paste for bone tissue engineering.

Ping Wang1, Yang Song2, Michael D Weir2

  • 1Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA; State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China.

Dental Materials : Official Publication of the Academy of Dental Materials
|January 9, 2016
PubMed
Summary
This summary is machine-generated.

Injectable calcium phosphate cements (CPCs) with microbead-encapsulated induced pluripotent stem cell-derived mesenchymal stem cells (iPSMSCs) significantly enhanced bone regeneration in rats. Pre-osteoinduced or BMP2-modified iPSMSCs showed superior bone formation and accelerated scaffold resorption compared to controls.

Keywords:
Alginate microbeadsAnimal studiesBone tissue engineeringCalcium phosphate cementHuman induced pluripotent stem cellsInjectable

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Area of Science:

  • Biomaterials Science
  • Stem Cell Biology
  • Regenerative Medicine

Background:

  • Calcium phosphate cements (CPCs) are widely investigated for bone defect repair.
  • Injectable delivery systems are desirable for minimally invasive procedures.
  • Encapsulating cells within microbeads offers a promising strategy for cell delivery in tissue engineering.

Purpose of the Study:

  • To develop an injectable cell delivery system using microbead-encapsulated induced pluripotent stem cell-derived mesenchymal stem cells (iPSMSCs).
  • To create a novel tissue-engineered construct by incorporating iPSMSC-laden microbeads into CPC.
  • To evaluate the efficacy of this construct in promoting bone regeneration in an animal model.

Main Methods:

  • iPSMSCs were pre-osteoinduced (OS-iPSMSCs) or transduced with bone morphogenetic protein-2 (BMP2-iPSMSCs).
  • Cells were encapsulated in fast-degradable alginate microbeads and mixed with CPC paste.
  • The constructs were implanted into cranial defects in nude rats, with control groups receiving CPC-microbeads without cells.

Main Results:

  • Encapsulated cells maintained viability and exhibited osteogenic marker upregulation.
  • New bone formation was significantly higher in groups with OS-iPSMSCs (38.9±18.4%) and BMP2-iPSMSCs (44.7±22.8%) compared to CPC control (15.6±11.2%).
  • Cell-laden CPC constructs accelerated scaffold resorption, with the BMP2-iPSMSCs group showing 7-fold less remaining material than controls at 12 weeks.

Conclusions:

  • Injectable CPC-microbead-iPSMSC constructs effectively promote bone regeneration.
  • Pre-osteoinduction and BMP2 modification of iPSMSCs enhance new bone formation.
  • This approach represents a promising injectable material for orthopedic, dental, and craniofacial bone regeneration applications.