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Related Concept Videos

Fixation and Sectioning01:03

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Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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Related Experiment Video

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Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex
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An improved approach to align and embed multiple brain samples in a gelatin-based matrix for simultaneous

Kumi Nagamoto-Combs1, Gunjan D Manocha2, Kendra Puig2

  • 1Department of Pathology, University of North Dakota School of Medicine and Health Sciences, 501N. Columbia Road Stop 9037, Grand Forks, ND 58202-9037, USA.

Journal of Neuroscience Methods
|January 9, 2016
PubMed
Summary

This study introduces a new method using a template to easily embed multiple rodent brains in a gelatin matrix. This simplifies histological processing, allowing for simultaneous sectioning and mounting of samples.

Keywords:
Brain arrayEmbeddingFrozen-sectionGelatinImmunohistochemistryMatrixTissue preparation

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Area of Science:

  • Histology
  • Neuroscience
  • Biotechnology

Background:

  • Histological sectioning and mounting labor intensifies with increased sample numbers.
  • Embedding tissues in a flexible matrix aids simultaneous handling and preserves integrity.
  • Aligning asymmetrical samples like rodent brains requires skill and specialized techniques.

Purpose of the Study:

  • To describe an improved method for aligning and embedding multiple rodent brain samples.
  • To simplify the preparation of gelatin-embedded brain arrays for histological processing.

Main Methods:

  • Developed a template to create mouse brain-shaped cavities in a 'receiving matrix'.
  • Inserted whole or half rodent brains directly into the cavities for uniform orientation.
  • Embedded samples within a gelatin-based matrix without manual pinning.

Main Results:

  • Successfully arrayed multiple mouse brains in uniform orientation within a gelatin matrix.
  • Demonstrated effortless sectioning, staining, and mounting of embedded samples onto slides.
  • Prevented sample damage or shifting during the embedding process.

Conclusions:

  • The novel approach simplifies the preparation of high-quality, gelatin-embedded whole or half brain arrays.
  • This method offers a commercially viable solution for processing multiple brain samples efficiently.